Transcriptions
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certain time of human development office and that's the most important organ if the placenta cannot really go into the
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supply of nutrients and or oxygen we are talking about
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essential in sufficiency finally leading to ensure to improve education
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and our objective was to assess what the effects of type oxygen starvation are on to sentence has
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and that's why we use to remotes less placenta cell and object three inch ourselves
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either in expose them to new i'm marks yeah which means zero point two
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percent of oxygen or room here and then either start them them way that um
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uh_huh them with or without the colours edition then
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we assessed viability d. n. a. content and thirteen amounts
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and on the red plot you see it after after the uh
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results of viability of the four hours eight hours and twenty four hours
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and the first lot the uh circles in white it's normal to occur
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then comes high plucks yeah then normal to us that asian and white box yes their patient
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and it's easily visible that type talks yeah alone does not really affect my ability but that
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not hard box yeah installations finally
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pizzas to a significant decrease of viability
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um so you see the image after twenty four hours object ourselves on
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the left and you see it nicely the it rolls off the cells in
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here to write down below button a ways fact box yeah starvation in combination
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and it's similar to is uh due to d. n. a. contents
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sell 'em it the longer the cells are exposed to high any
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'cause the patient the more reach use the d. n. a. content is this is
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also true for protein amount which is reduced to one fourth after twenty four hours
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up to now we haven't um detect it it's signalling component being responsible for
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that's and that's why we will for the s. s. if
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this is the closest up hoses or to a g. going on
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and ah question it's
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this is a very good question because we know that the children uh are it's a bit details is
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kind of a yeah four thousand c. level as so we do not um
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directly know what the exact oxygen amount should be in the cellars
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so even within the class and i probably there is a gradient accent
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but for sure zero point two percent is new and knock sick
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so this is not uh the the stitches they normally should have
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it is quite difficult to assess what what is the normal condition
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good because you you don't compare normal to be possible to put part boxer would type provokes your costume
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one hundred and fifty you mixed work review to disprove influence these results
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or some two different groups so we discuss whether we should
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probably then incubate is it directly at the lower amount of oxygen
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to assume that there is a link with layer covered on top
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of the cells which also in here the oxygen transfers we then decided
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to go oh or this easy approach in order to know what yeah yeah what